A Radioimmunoassay for Urinary Estrogens
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of total urinary estrogens.
We describe a radioimmunoassay for total estrogens in urine. Estrogens are extracted by adsorption on XAD-2 resin, eluted with methanol, and acid-hydrolyzed. Estrogens are then determined in a diluted aliquot of the hydrolysate. Within-run coefficients of variation for estriol values between 6.0 and 100 micrograms/L were less than 5%; between-run CV was 19.6% at an estriol concentration of 16.8...
متن کاملMeasurement of urinary estrogens.
In a commentary on the existingmethodology for the determination of urinary estrogens, data are givenfor normalrangesin nonpregnancy and pregnancyurine aswell as the expectedinterferencesdue to medicationand substancesoccurring in high concentrationsin diseasestates. Becauseof the wide rangeof normal excretionvalues,the point is madethat onlyserialanalysesof a patient’sphysiologic course (e.g.,...
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We describe a radioimmunoassay for lysozyme (EC 3.2.1.17, mucopeptide N-acetylmuramohydrolase) in plasma and urine. It involves use of an antibody, raised in rabbits, to lysozyme from urine from a patient with monocytic leukemia. This antiserum, diluted 4000-fold, is incubated with radiolabeled lysozyme for 2 h and antibody-bound lysozyme is separated from free lysozyme with dextran-coated char...
متن کاملRadioimmunoassay for free and conjugated urinary metanephrine.
We describe a convenient and specific radioimmunoassay for urinary metanephrine, in which we used an antiserum generated in rabbits by injecting with synephrine conjugated to bovine serum albumin as described by Grota and Brown [Endocrinology 98, 615 (1976)]. The antiserum is specific for metanephrine and epinephrine. Epinephrine cross reaction is minimized to 1% by adding 2 ng of epinephrine t...
متن کاملSimple assay for urinary estrogens in nonpregnancy.
Shackleton et al. (1), Osawa and Siaunwhite 2), and Beckloff et al. (3) have recently reported simple methods for assay of steroid conjugates in urine by use of columns of Amberlite XAD-2. With Osawa and Slaunwhite’s procedure urinary estrogen conjugates can be assayed with a lower limit of detection in the micromolar range but, as implied by the authors, the assay is useful only for analysis o...
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ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1972
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.48.5_324